CAT #: 23080010
BCL1/JH Tube - Unlabeled for Gel Detection
BCL1/JH Tube – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify IGH-CCND1 (BCL1) translocations.
The aberrant BCL1/JH t(11;14) translocation juxtaposes genes of the immunoglobulin heavy chain (IGH) gene on chromosome 14q32 with the cyclin D1 gene on chromosome 11q13. The juxtaposition of IGH-sequences results in the transcriptional activation of cyclin D1.1,2 Cyclin D1 is involved in the regulation of the G1 progression and G1/S transition of the cell cycle.3 Translocation does not lead to expression of a fusion protein. In fact, oncogenesis is due to a promoter/enhancer exchange, wherein the immunoglobulin gene enhancer stimulates the expression of cyclin D1. Overexpression of cyclin D1, in turn, accelerates passage of transformed cells through the G1 phase.
The BCL1/JH Tube master mix targets the major translocation cluster (MTC) of the BCL1 and IG heavy chain locus. PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium staining.
1. van Dongen, JJM, et. al., (2003). Leukemia. 17(12):2257-2317.
2. Janssen, J.W.G., et. al., (2000). Blood. 95:2691-2698.
3. Shimazaki C, et. al., (1997). International Journal of Hematology. 66(1):111-5.
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