CAT #: 14120029
FLT3 ITD MRD v1.2 Software for Use on Illumina® MiSeqTM Platform
The FLT3 ITD MRD v1.2 Software package provides automated bioinformatic analyses of raw FASTQ files generated by the Illumina® MiSeqTM, when used with the FLT3 ITD MRD Assay. The dockerized Linux-based software is built for portability, flexibility and can be easily integrated into down-stream reporting systems and/or LIMS.
The FLT3 ITD MRD Assay for use with the Illumina® MiSeqTM platform was designed to produce sequencing data that can be analyzed using the FLT3 ITD MRD v1.2 software package provided on the associated USB (Catalog #14120029). This USB includes detailed README files with instructions for installation and use of the software package (using either the command-line or REST-API execution method), as well as a MiSeqTM sample sheet template.
The MiSeqTM sequencing run of samples prepared with the FLT3 ITD MRD Assay outputs FASTQ files that can be easily processed by the FLT3 ITD MRD v1.2 Software to provide FLT3 ITD variant calls. Individual sample and aggregate sample data output is generated in the form of .tsv (tab-separated value) files. This software package allows for the identification and tracking of internal tandem duplications (ITD) in the fms-like tyrosine kinase 3 (FLT3) gene and provides the length of the ITD up to 252 base pairs. Additionally, counts and nucleotide sequences for each FLT3-aligned amplicon can be accessed via the <sample>.merge.seq file of the run folder.
This software reports ITD results as:
- Not Detected
Variant Read Frequency (VRF) and confidence for the detection of an ITD with an input Variant Allele Frequency (VAF) of 5×10-5 is reported for Positive and Not Detected results.
This product is for Research Use Only; not for use in diagnostic procedures.
Use of this product may require nucleic acid amplification methods such as Polymerase Chain Reaction (PCR). Any necessary license to practice amplification methods or to use reagents, amplification enzymes or equipment covered by third party patents is the responsibility of the user and no such license is granted by Invivoscribe, Inc., expressly or by implication.
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