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CAT #: 13080010

BCL1/JH Translocation Assay for Gel Detection

Assay Use

This Research Use Only assay identifies BCL1/JH t(11;14) translocations and is useful for the evaluation of new research and methods in malignancy studies.

Product Details

  • Summary and Explanation of the Test

    BACKGROUND
    This aberrant BCL1/JH t(11;14) translocation juxtaposes genes of the immunoglobulin heavy chain (IGH) gene on chromosome 14q32 with the cyclin D1 gene on chromosome 11q13.  The juxtaposition of IGH-sequences results in the transcriptional activation of cyclin D1.1,2  Cyclin D1 is involved in the regulation of the G1 progression and G1/S transition of the cell cycle.3  Translocation does not lead to expression of a fusion protein.  In fact, oncogenesis is due to a promoter/enhancer exchange, wherein the immunoglobulin gene enhancer stimulates the expression of cyclin D1.  Overexpression of cyclin D1, in turn, accelerates passage of transformed cells through the G1 phase.

    SUMMARY
    Invivoscribe’s gene rearrangement and translocation assays represent a simple approach to PCR-based clonality testing.  These standardized assays were carefully optimized testing positive and negative control samples using multiplex master mixes.  Included in this test kit are two (2) master mixes.  The BCL1/JH master mix targets the major translocation cluster (MTC) of the BCL1 locus and the joining region of the Ig heavy chain locus.  The other master mix, the Specimen Control Size Ladder, targets multiple genes and generates a series of amplicons of 100, 200, 300, 400, and 600 base pair (bp) that can be used to ensure that the quality and quantity of input DNA is adequate to yield a valid result.

  • Specimen Requirements

    This assay tests extracted and purified genomic DNA (gDNA). Common sources of gDNA include:

    • 5 cc of peripheral blood, bone marrow biopsy or bone marrow aspirate anti-coagulated with heparin or EDTA. Ship at ambient temperature; OR
    • Formalin-fixed paraffin embedded tissue or slides.

References

1. van Dongen, JJM, et. al. (2003) Leukemia. 17(12):2257-2317.
2. Janssen, JWG, et. al.,(2000) Blood. 95:2691-2698.
3. Shimazaki C, et. al., (1997) International Journal of Hematology. 66(1):111-5.

Disclaimer

This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.

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