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CAT #: 22050031

TCRB Tube C - 6FAM & HEX for ABI Fluorescence Detection

Product Use

TCRB Tube C – 6FAM & HEX for ABI Fluorescence Detection is a Research Use Only master mix that can be used to identify T-cell receptor beta (TCRB) gene rearrangements.

Product Details

  • Description

    Rearrangements of the antigen receptor genes occur during ontogeny in B and T lymphocytes.  These gene rearrangements generate products that are unique in length and sequence for each cell.  Therefore, polymerase chain reaction (PCR) assays can be used to identify lymphocyte populations derived from a single cell by detecting the unique V-J gene rearrangements present within these antigen receptor loci.  The human TCR beta gene locus on chromosome 7 (7q35) includes 64-67 V genes belonging to 32 subgroups, 2 D segments and 13 J segments, spread over 620 kilobases.

    This master mix targets conserved conserved regions within the variable (Vβ), diversity (Dβ), and the joining (Jβ) regions that flank the unique hypervariable, antigen-binding, and complementarity determining region 3 (CDR3).  TCRB Tube C contains 2 Dβ primers and 13 Jβ primers.  Clonality is indicated if the master mix generates clonal band(s).  PCR products are differentially labeled with 6FAM & HEX and can be analyzed using any platform that has the ability to detect these fluorochromes.

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