Product Availability Key

  • Mexico and Canada flagMexico and Canada
  •  Outside North America flag Outside North America
  •  US flag US
  • Mexico and Canada flag
  •  Outside North America flag
  •  US flag

CAT #: 22050011

TCRB Tube A - 6FAM & HEX for ABI Fluorescence Detection

Product Use

TCRB Tube A – 6FAM & HEX for ABI Fluorescence Detection is a Research Use Only master mix that can be used to identify T-cell receptor beta (TCRB) gene rearrangements.

Product Details

  • Description

    Rearrangements of the antigen receptor genes occur during ontogeny in B and T lymphocytes.  These gene rearrangements generate products that are unique in length and sequence for each cell.  Therefore, polymerase chain reaction (PCR) assays can be used to identify lymphocyte populations derived from a single cell by detecting the unique V-J gene rearrangements present within these antigen receptor loci.  The human TCR beta gene locus on chromosome 7 (7q35) includes 64-67 V genes belonging to 32 subgroups, 2 D segments and 13 J segments, spread over 620 kilobases.

    This master mix targets conserved conserved regions within the variable (Vβ), diversity (Dβ), and the joining (Jβ) regions that flank the unique hypervariable, antigen-binding, and complementarity determining region 3 (CDR3).  TCRB Tube A contains 23 Vβ primers, 6 Jβ1 primers, and 3 Jβ2 primers.  Clonality is indicated if the master mix generates clonal band(s).  PCR products are differentially labeled with 6FAM & HEX and can be analyzed using any platform that has the ability to detect these fluorochromes.

Legal Notice

Now Available

Our New Document Search Feature
SEARCH ⟶

Need Help Placing an Online Order?

Contact our Customer Service Team
CONTACT CUSTOMER SERVICE ⟶

Now Available

Our New Document Search Feature
SEARCH ⟶

Need Help Placing an Online Order?

Contact our Customer Service Team
CONTACT CUSTOMER SERVICE ⟶