CAT #: 22050011
TCRB Tube A - 6FAM & HEX for ABI Fluorescence Detection
TCRB Tube A – 6FAM & HEX for ABI Fluorescence Detection is a Research Use Only master mix that can be used to identify T-cell receptor beta (TCRB) gene rearrangements.
Rearrangements of the antigen receptor genes occur during ontogeny in B and T lymphocytes. These gene rearrangements generate products that are unique in length and sequence for each cell. Therefore, polymerase chain reaction (PCR) assays can be used to identify lymphocyte populations derived from a single cell by detecting the unique V-J gene rearrangements present within these antigen receptor loci. The human TCR beta gene locus on chromosome 7 (7q35) includes 64-67 V genes belonging to 32 subgroups, 2 D segments and 13 J segments, spread over 620 kilobases.
This master mix targets conserved conserved regions within the variable (Vβ), diversity (Dβ), and the joining (Jβ) regions that flank the unique hypervariable, antigen-binding, and complementarity determining region 3 (CDR3). TCRB Tube A contains 23 Vβ primers, 6 Jβ1 primers, and 3 Jβ2 primers. Clonality is indicated if the master mix generates clonal band(s). PCR products are differentially labeled with 6FAM & HEX and can be analyzed using any platform that has the ability to detect these fluorochromes.
Warranty and Liability
Invivoscribe, Inc. (Invivoscribe®) is committed to providing the highest quality products. Invivoscribe® warrants that the products meet or exceed the performance standards described in the Instructions For Use, as to products with such an insert. If a product is covered by product specifications and does not perform as specified, our policy is to replace the product or credit the full purchase price. No other warranties of any kind, expressed or implied, are provided by Invivoscribe®. Invivoscribe® liability shall not exceed the purchase price of the product. Invivoscribe® shall have no liability for direct, indirect, consequential or incidental damages arising from the use, results of use, or inability to use its products; product efficacy under purchaser controlled conditions in purchaser’s laboratory must be established and continually monitored through purchaser defined and controlled processes including but not limited to testing of internally validated positive, negative, and blank controls every time a sample is tested. Ordering, acceptance and use of product constitutes purchaser acceptance of sole responsibility for assuring product efficacy and purchaser agreement to the limitation of liability set forth in this paragraph.
This product is for Research Use Only; not for use in diagnostic procedures.
©2023 Invivoscribe, Inc. All rights reserved. The trademarks mentioned herein are the property of Invivoscribe, Inc. and/or its affiliates, or (as to the trademarks of others used herein) their respective owners.