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CAT #: 20960020

Specimen Control Size Ladder - Unlabeled for Gel Detection

Assay Use

The General Purpose Reagent (GPR) Specimen Control Size Ladder master mix can be used to ensure that the quantity and quality of unknown sample DNA is adequate to yield a valid result during molecular testing.

Product Details

  • Summary and Explanation of the Test

    Sample quality is critical and therefore it must be verified before the samples are used in molecular assays. Analysis of degraded DNA samples can result in poor quality data, and the presence of inhibitors differentially affects qPCR assays.1,2  Therefore, verification of the quantity and quality of the sample is sufficient for the molecular assay is important, and one method is to employ an amplification control master mix.

    Master mixes are components of complete assays and typically consist of a buffered magnesium solution, deoxynucleotides, and multiple primers that target the gene segments of interest.  Multiple primers are used to ensure a more comprehensive testing approach necessary to reliably identify clonal rearrangements.  This test is complete with the exception of Taq DNA polymerase, which is not provided.

    The Specimen Control Size Ladder master mix is included in many of our testing kits and targets four different genes: Tbxas (thromboxane synthase), RAG-1 (recombination activating gene-1), PLZF (promyelocytic leukemia zinc finger) and AF4 (ALL1 fused gene from chromosome 4).  Amplification of genomic DNA (gDNA) with this master mix produces products of 100, 200, 300, 400, and 600 base pairs (bp) to ensure that the quality and quantity of the sample DNA is adequate to yield a valid result.

  • Specimen Requirements

    This master mix tests genomic DNA (gDNA) from the following sources:

    • 5 cc of peripheral blood, bone marrow biopsy or bone marrow aspirate anti-coagulated with heparin or EDTA. Ship at ambient temperature; OR
    • Minimum 5 mm cube of tissue shipped frozen; or at room temperature or on ice in RPMI 1640; OR
    • 2 µg of gDNA; OR
    • Formalin-fixed paraffin embedded tissue or slides

References

1.  Vermeulen J et al. (2011) Nucleic Acids Research. May;39(9):e63.
2.  Huggett JF et al. (2008) BMC Research Notes. Aug 28;1:70.

Disclaimer

This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.

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