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CAT #: S100005

LeukoStrat® FLT3-ITD MRD Software

Product Use

The LeukoStrat® FLT3-ITD MRD Software package provides automated bioinformatic analyses of raw FASTQ files generated by the Illumina® MiSeqTM, MiSeqTM i100, NextSeqTM 1000 and Complete Genomics DNBSEQ- G99 when used with the LeukoStrat® FLT3-ITD MRD Assay. The dockerized Linux-based software is built for portability, flexibility and can be easily integrated into down-stream reporting systems and/or LIMS.

Product Details

  • Description

    The LeukoStrat® FLT3-ITD MRD Assay was designed to produce sequencing data that can be analyzed using the LeukoStrat® FLT3-ITD MRD Software package. This includes detailed README files with instructions for installation and use of the software (using either the command-line or REST-API execution method).

    The sequencing run of samples prepared with the LeukoStrat® FLT3-ITD MRD Assay outputs FASTQ files that can be easily processed by the LeukoStrat® FLT3-ITD MRD Software to provide FLT3-ITD variant calls. Individual sample and aggregate sample data output is generated in the form of .tsv (tab-separated value) files. This software package allows for the identification and tracking of internal tandem duplications (ITD) in the fms-like tyrosine kinase 3 (FLT3) gene and provides the length of the ITD up to 252 base pairs.

    This software reports ITD results as:

    • Positive
    • Not Detected
    • Undetermined

    Variant Read Frequency (VRF) and confidence for the detection of an ITD with an input Variant Allele Frequency (VAF) of 5×10-5 is reported for Positive and Not Detected results.

    System Requirements:

    • Operating System: Linux (Ubuntu/RedHat with Docker installed).
    • Containerization: Docker.
    • Sequencers: Illumina (MiSeqTM, MiSeqTM i100, NextSeqTM 1000), Complete Genomics DNBSEQ-G99.
    • Processor: Minimum of 1 processor for processing MiSeqTM, MiSeqTM i100, NextSeqTM 1000, and DNBSEQ-G99 datasets. 16 processors or more recommended for MiSeqTM, MiSeqTM i100, and DNBSEQ-G99. 64 processors or more recommended for NextSeqTM 1000.
    • Disk: 12 GB for installation of the software. 50 GB of available empty space for processing a set of MiSeqTM, MiSeq i100 or DNBSEQ-G99 FASTQ files, and 100 GB for NextSeqTM 1000.
    • RAM: 8 GB required; 32 GB or more recommended for MiSeqTM, MiSeqTM i100, and DNBSEQ-G99. 24 GB required; 256 GB or more recommended for NextSeqTM 1000 datasets.

Disclaimer

This product is for Research Use Only; not for use in diagnostic procedures.

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