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CAT #: 13090040

BCL2/JH Translocation Assay MegaKit - Gel Detection

Product Use

BCL2/JH Translocation Assays are useful for the study of:

  • Monitoring and evaluation of follicular lymphomas and other B cell lymphomas
  • Distinguishing lymphoma from benign lymphoid hyperplasia
  • Distinguishing follicular lymphoma from other B cell lymphomas that may have a similar appearance
  • Monitoring and evaluation of disease recurrence
  • Detection and assessment of residual disease
  • Evaluation of new research and methods in malignancy studies

Product Details

  • Summary of Explanation of the Test

    SUMMARY:

    Invivoscribe’s Gene Rearrangement and Translocation Assays represent a new approach to PCR-based clonality testing. These standardized assays were carefully optimized testing positive and negative control samples using multiplex master mixes.

    BACKGROUND:

    BCL2 translocations are reciprocal chromosome exchanges that place the bcl-2 proto-oncogene, located on chromosome 18, under aberrant transcriptional control of the immunoglobulin heavy chain gene, located on chromosome 14. The bcl-2 protein is an antagonist to apoptosis (programmed cell death), a normal process designed to eliminate unneeded and damaged cells during hematopoiesis. Increased expression of the bcl-2 protein leads to an increase in the levels of B cells in the body. BCL2 t(14;18) translocations are present in 70-90% of follicular non-Hodgkin B cell lymphomas, 50% of undifferentiated B cell lymphomas, and 20-30% of large cell diffuse B cell lymphomas. They are not seen in other lymphomas; therefore, this test is useful for the differential findings of B cell malignancies. Further, presence of the bcl-2 translocation is an indicator of poor prognosis in large cell diffuse B cell lymphomas.

    This BCL2 t(14;18) Translocation Assay contains four master mixes. Three of the master mixes target the joining region of the immunoglobulin heavy chain gene, and distinct regions of the BCL2 gene. These master mixes are used to detect major breakpoint region (Mbr) and minor cluster region (mcr) of the BCL2 t(14;18) translocations. The fourth master mix, the Specimen Control Size Ladder, targets multiple genes and generates a series of amplicons of 100, 200, 300, 400, and 600 base pairs to ensure that the quality and quantity of input DNA is adequate to yield a valid result. Positive and negative control DNAs and also included. This assay is used both to identify and monitor BCL2 translocations involving the major breakpoint region (Mbr) and the minor cluster region (mcr). The assay can also be used to detect disease recurrence.

  • Specimen Requirements

    This assay tests genomic DNA

    • 5cc of peripheral blood, bone marrow biopsy, or bone marrow aspirate anti-coagulated with heparin or EDTA. Ship at ambient temperature; OR
    • Minimum 5mm cube of tissue shipped frozen; or at room temperature or on ice in RPMI 1640; OR
    • 2µg of genomic DNA; OR
    • Formalin-fixed paraffin embedded tissue or slides.

Disclaimer

This product is sold FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

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