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CAT #: 13090020

BCL2/JH Translocation Assay for Gel Detection

Assay Use

This Research Use Only assay identifies BCL2/JH t(14;18) translocations.

Product Details

  • Summary and Explanation of the Test

    BACKGROUND
    BCL2 translocations are reciprocal chromosome exchanges that place the bcl-2 proto-oncogene, located on chromosome 18, under aberrant transcriptional control of the immunoglobulin heavy chain gene, located on chromosome 14.  The bcl-2 protein is an antagonist to apoptosis (programmed cell death), a normal process designed to eliminate unneeded and damaged cells during hematopoiesis.  Increased expression of the bcl-2 protein leads to an increase in the levels of B-cells in the body.

    SUMMARY
    This BCL2 t(14;18) Translocation Assay contains four (4) master mixes.  Primers in three (3) of the master mixes target the joining region of the immunoglobulin heavy chain gene and distinct regions of the BCL2 gene.  These master mixes are used to detect major breakpoint region (Mbr) and minor cluster region (mcr) of the BCL2 t(14;18) translocations.  The remaining master mix, the Specimen Control Size Ladder, targets multiple genes and generates a series of amplicons of 100, 200, 300, 400, and 600 base pairs (bp) to ensure DNA of sufficient quality and quantity was present to generate a valid result.  Positive and negative control DNA are also included.

     

  • Specimen Requirements

    This assay tests genomic DNA.

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