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CAT #: 22070030

TCRG Tube A - Unlabeled for Gel Detection

Product Use

The TCRG Tube A – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify T-cell receptor gamma chain gene rearrangements.

Product Details

  • Description

    The T-cell receptor gamma (TCRG) chain locus spans 160 kb on chromosome 7 (7p14).  Rearrangement of the variable (Vγ) and joining (Jγ) genetic segments of the TCRG locus result in Vγ-Jγ products of unique length and sequence.  The TCRG locus does not contain D segments.  Additionally, the TCRG gene contains a limited number of Vγ and Jγ segments such that the amplification of all major Vγ-Jγ combinations is possible with four Vγ and two Jγ primers.

    This master mix targets conserved regions within the variable (Vγ) and joining (Jγ) regions that flank the unique, hypervariable, antigen-binding, complementarity determining region 3 (CDR3), containing two (2) Vγ primers and two (2) Jγ primers.  Clonality is indicated if any clonal band(s) are generated.  PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium staining.

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