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CAT #: 12070021

TCRG Gene Clonality Assay for ABI Fluorescence Detection

Assay Use

This Research Use Only assay identifies T-cell receptor gamma chain gene rearrangements and is useful for the study of clonal T-cell populations and evaluation of new research and methods in malignancy studies.

Product Details

  • Summary and Explanation of the Test

    BACKGROUND
    The human TCR gamma gene locus on chromosome 7 (7q14) includes 14 variable (V) genes belonging to four (4) subgroups (six (6) are functional; three (3) Open Reading Frames and five (5) pseudogenes), five (5) J segments and two (2) C genes spread over 200 kilobases.  However, this standardized multiplex PCR assay detects the vast majority of clonal TCR gamma gene rearrangements using only two (2) multiplex master mixes.

    SUMMARY
    Invivoscribe’s gene rearrangement and translocation assays represent a simple approach to PCR-based clonality testing.  These standardized assays were carefully optimized testing positive and negative control samples using multiplex master mixes.  This test kit includes three master mixes.  Tubes A and B target framework regions within the variable (V) region and the joining (J) region of the TCR gamma chain locus.  The Specimen Control Size Ladder master mix targets multiple genes and generates a series of amplicons of 100, 200, 300, 400, and 600 base pair (bp) to ensure the quality and quantity of input DNA is adequate to yield a valid result.

     

  • Specimen Requirements

    This assay tests genomic DNA.

Disclaimer

This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.

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