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CAT #: 22060010

TCRD Tube - Unlabeled for Gel Detection

Product Use

TCRD Tube – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify T-cell receptor delta (TCRD) gene rearrangements.

Product Details

  • Description

    The human T-cell receptor delta (TCRD) gene locus is comprised of a cluster of 10 genes located on chromosome 14 (14q11.2) spread over 60 kilobases, localized between the T-cell receptor alpha (TCRA) variable (Vα) and joining (Jα) gene segments.  It is made up of eight (8) variable (Vδ), three (3) diversity (Dδ), and four (4) joining (Jδ) gene segments.  At least five (5) of the eight (8) Vδ gene segments can also rearrange to Jα gene segments and other Vα gene segments may also be utilized in TCRD gene rearrangements in rare cases.  Although the small number of Vδ, Dδ, and Jδ gene segments available for recombination limits the potential combinatorial diversity, the complementarity determining region 3 (CDR3) or junctional diversity is extensive due to the addition of N regions, P regions, and random deletion of nucleotides by recombinases.  This diversity is also extended by the recombination of up to three Dδ segments and therefore up to four N regions within the rearranged TCRD locus.  This limited germline diversity encoded at the TCRD locus in conjunction with extensive junctional diversity results in a useful target for PCR analysis.

    The TCRD Tube master mix targets conserved regions within the variable (Vδ1-6), the diversity (Dδd2-3) and the joining (Jδ1-4) regions that flank the unique, hypervariable, antigen-binding, complementarity determining region 3 (CDR3).  Clonality is indicated if the master mix generates clonal band(s).  PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium staining.

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