CAT #: 22050010
TCRB Tube A - Unlabeled for Gel Detection
TCRB Tube A – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify T-cell receptor beta (TCRB) gene rearrangements.
Rearrangements of the antigen receptor genes occur during ontogeny in B and T lymphocytes. These gene rearrangements generate products that are unique in length and sequence for each cell. Therefore, polymerase chain reaction (PCR) assays can be used to identify lymphocyte populations derived from a single cell by detecting the unique V-J gene rearrangements present within these antigen receptor loci. The human TCR beta gene locus on chromosome 7 (7q35) includes 64-67 V genes belonging to 32 subgroups, 2 D segments and 13 J segments, spread over 620 kilobases.
This master mix targets conserved conserved regions within the variable (Vβ), diversity (Dβ), and the joining (Jβ) regions that flank the unique hypervariable, antigen-binding, and complementarity determining region 3 (CDR3). TCRB Tube A contains 23 Vβ primers, 6 Jβ1 primers, and 3 Jβ2 primers. Clonality is indicated if the master mix generates clonal band(s). PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium staining.
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This product is for Research Use Only; not for use in diagnostic procedures.
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