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CAT #: 12000011

TCRB + TCRG T-Cell Clonality Assay for ABI Fluorescence Detection

Product Use

This Research Use Only assay uses PCR to detect clonal T-cell receptor beta and gamma chain gene rearrangements and is useful for the study of clonal T-cell populations and evaluation of new research and methods in malignancy studies.

Product Details

  • Summary and Explanation of the Test

    BACKGROUND
    Rearrangements of the antigen receptor genes occur during ontogeny in B and T lymphocytes.  These gene rearrangements generate products that are unique in length and sequence for each cell.  Therefore, polymerase chain reaction (PCR) assays can be used to identify lymphocyte populations derived from a single cell by detecting the unique V-J gene rearrangements present within these antigen receptor loci.  This assay employs multiple consensus DNA primers that target conserved genetic regions within the T-cell receptor beta chain and gamma chain genes.  This test is used to detect the vast majority of clonal T-cell malignancies from DNA. Test products can be analyzed using a variety of detection formats, including gel and capillary electrophoresis.

    SUMMARY
    Invivoscribe’s gene rearrangement and translocation assays represent a simple approach to PCR-based clonality testing.  These standardized assays were carefully optimized testing positive and negative control samples using multiplex master mixes.  This test kit includes six (6) master mixes.  TCRB Tubes A and B target framework regions within the variable region and the joining region of the TCR beta chain locus.  TCRB Tube C targets the diversity and joining regions.  TCRG Tube A contains primers that target the Vγ1-8 + Vγ10 genes and Jγ1.1, Jγ1.3, Jγ2.1 and Jγ2.3 genes (also known as JγP1, Jγ1, JγP2 and Jγ2 respectively).  TCRG Tube B contains primers that target the Vγ9 + Vγ11 genes and Jγ1.1, Jγ1.3, Jγ2.1 and Jγ2.3 genes.  Lastly, the Specimen Control Size Ladder, targets multiple genes and generates a series of amplicons approximately 100, 200, 300, 400, and 600 base pairs to ensure that the quality and quantity of input DNA is adequate to yield a valid result.  A single thermal cycler program and similar detection methodologies are used with all our Gene Clonality assays.  This improves consistency and facilitates cross training on a broad range of different assays.

  • Specimen Requirements

    This assay tests genomic DNA.

Disclaimer

This assay is based on the EuroClonality/BIOMED-2 Concerted Action BMH4-CT98-3936.

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