CAT #: 21020020
IGK Tube B - Unlabeled for Gel Detection
IGK Tube B – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify immunoglobulin kappa (IGK) light chain gene rearrangements.
The human immunoglobulin kappa (IGK) light chain locus on the short arm of chromosome 2 (2p11.2) spans 1820 kb. It is made up of 76 variable (Vκ) gene segments belonging to 7 subgroups, 5 joining (Jκ) gene segments, and one constant (Cκ) gene segment. Productive assembly of the kappa gene is successful in about 60% of human B lymphocytes. However, even when unsuccessful, clonal B-cells generally retain the rearranged kappa genes. The Vκ segments encode the first 95 N-terminal amino acids. Positions 96-108 are encoded by one of five joining (Jκ) gene segments. The constant (Cκ) portion of the kappa light chain (amino acids 109-214) is encoded by a single constant (Cκ) region separated from the Jκ region by an intron. The length of the hypervariable complementarity determining region 3 (CDR3) in kappa light chain genes is limited and rearrangements in this region display significant skewing (platykurtosis).
This master mix targets conserved regions within the variable (Vκ1-7) and the joining (Jκ1-5) regions that flank the unique hypervariable, antigen-binding, complementarity determining region 3 (CDR3) as well as the Kde and intragenic regions. IGK Tube B contains six upstream Vκ region primers, an upstream intragenic primer and a downstream Kde primer. Clonality is indicated if the master mix generates clonal band(s). PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium staining.
Warranty and Liability
Invivoscribe, Inc. (Invivoscribe®) is committed to providing the highest quality products. Invivoscribe® warrants that the products meet or exceed the performance standards described in the Instructions For Use, as to products with such an insert. If a product is covered by product specifications and does not perform as specified, our policy is to replace the product or credit the full purchase price. No other warranties of any kind, expressed or implied, are provided by Invivoscribe®. Invivoscribe® liability shall not exceed the purchase price of the product. Invivoscribe® shall have no liability for direct, indirect, consequential or incidental damages arising from the use, results of use, or inability to use its products; product efficacy under purchaser controlled conditions in purchaser’s laboratory must be established and continually monitored through purchaser defined and controlled processes including but not limited to testing of internally validated positive, negative, and blank controls every time a sample is tested. Ordering, acceptance and use of product constitutes purchaser acceptance of sole responsibility for assuring product efficacy and purchaser agreement to the limitation of liability set forth in this paragraph.
This product is for Research Use Only; not for use in diagnostic procedures.
©2020 Invivoscribe, Inc. All rights reserved. The trademarks mentioned herein are the property of Invivoscribe, Inc. and/or its affiliates, or (as to the trademarks of others used herein) their respective owners.