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CAT #: 21020020

IGK Tube B - Unlabeled for Gel Detection

Product Use

IGK Tube B – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify immunoglobulin kappa (IGK) light chain gene rearrangements.

Product Details

  • Description

    DESCRIPTION
    The human immunoglobulin kappa (IGK) light chain locus on the short arm of chromosome 2 (2p11.2) spans 1820 kb.  It is made up of 76 variable (Vκ) gene segments belonging to 7 subgroups, 5 joining (Jκ) gene segments, and one constant (Cκ) gene segment.  Productive assembly of the kappa gene is successful in about 60% of human B lymphocytes.  However, even when unsuccessful, clonal B-cells generally retain the rearranged kappa genes.  The Vκ segments encode the first 95 N-terminal amino acids.  Positions 96-108 are encoded by one of five joining (Jκ) gene segments.  The constant (Cκ) portion of the kappa light chain (amino acids 109-214) is encoded by a single constant (Cκ) region separated from the Jκ region by an intron.  The length of the hypervariable complementarity determining region 3 (CDR3) in kappa light chain genes is limited and rearrangements in this region display significant skewing (platykurtosis).

    This master mix targets conserved regions within the variable (Vκ1-7) and the joining (Jκ1-5) regions that flank the unique hypervariable, antigen-binding, complementarity determining region 3 (CDR3) as well as the Kde and intragenic regions. IGK Tube B contains six upstream Vκ region primers, an upstream intragenic primer and a downstream Kde primer. Clonality is indicated if the master mix generates clonal band(s).  PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium staining.

     

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