CAT #: 21010050
IGH Tube E - Unlabeled for Gel Detection
IGH Tube E – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify immunoglobulin heavy chain clonality.
The immunoglobulin heavy chain (IGH) gene locus on chromosome 14 (14q32.3) includes 46-52 functional and 30 non-functional variable (VH) gene segments, 27 functional diversity (DH) gene segments, and 6 functional joining (JH) gene segments spread over 1250 kilobases. The most frequently used VH gene segments in normal and malignant B cells belong to the VH3, VH4, and VH1 family, together covering 75-95% of VH usage. The VH gene segments contain three framework regions (FR) and two complementarity determining regions (CDR). The FRs are characterized by their similarity among the various VH segments, whereas the CDRs are highly different even within the same VH family. The CDRs represent the preferred target sequences for somatic hypermutations; however, somatic mutations can also occur in the FRs. Therefore, family-specific primers in the three different FRs were designed to increase the detection rate of clonal IGH B-cell populations and decrease the occurrence of false-negative results due to somatic hypermutation in primer binding sites. In addition to VH-JH rearrangements, incomplete DHJH rearrangements have been found in mature and immature B-cell malignancies. Therefore, DH-JH PCR analysis may be of added value for clonality assessment.
This master mix targets conserved regions within the diversity (DH) and joining (JH) regions that flank the unique hypervariable, antigen-binding, complementarity determining region 3 (CDR3) and contains a DH7 region primer and a consensus JH primer. Clonality is indicated if the master mix generates clonal band(s). PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium staining.
Warranty and Liability
Invivoscribe, Inc. (Invivoscribe®) is committed to providing the highest quality products. Invivoscribe® warrants that the products meet or exceed the performance standards described in the Instructions For Use, as to products with such an insert. If a product is covered by product specifications and does not perform as specified, our policy is to replace the product or credit the full purchase price. No other warranties of any kind, expressed or implied, are provided by Invivoscribe®. Invivoscribe® liability shall not exceed the purchase price of the product. Invivoscribe® shall have no liability for direct, indirect, consequential or incidental damages arising from the use, results of use, or inability to use its products; product efficacy under purchaser controlled conditions in purchaser’s laboratory must be established and continually monitored through purchaser defined and controlled processes including but not limited to testing of internally validated positive, negative, and blank controls every time a sample is tested. Ordering, acceptance and use of product constitutes purchaser acceptance of sole responsibility for assuring product efficacy and purchaser agreement to the limitation of liability set forth in this paragraph.
This product is for Research Use Only; not for use in diagnostic procedures.
©2023 Invivoscribe, Inc. All rights reserved. The trademarks mentioned herein are the property of Invivoscribe, Inc. and/or its affiliates, or (as to the trademarks of others used herein) their respective owners.