CAT #: 21010020
IGH Tube B - Unlabeled for Gel Detection
IGH Tube B – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify immunoglobulin heavy chain clonality.
The immunoglobulin heavy chain (IGH) gene locus on chromosome 14 (14q32.3) includes 46-52 functional and 30 non-functional variable (VH) gene segments, 27 functional diversity (DH) gene segments, and 6 functional joining (JH) gene segments spread over 1250 kilobases. The most frequently used VH gene segments in normal and malignant B-cells belong to the VH3, VH4, and VH1 family, together covering 75-95% of VH usage. The VH gene segments contain three framework regions (FR) and two complementarity determining regions (CDR). The FRs are characterized by their similarity among the various VH segments, whereas the CDRs are highly different even within the same VH family. The CDRs represent the preferred target sequences for somatic hypermutations; however, somatic mutations can also occur in the FRs. Therefore, family-specific primers in the three different FRs were designed to increase the detection rate of clonal IGH B-cell populations and decrease the occurrence of false-negative results due to somatic hypermutation in primer binding sites. In addition to VH-JH rearrangements, incomplete DHJH rearrangements have been found in mature and immature B-cell malignancies. Therefore, DH-JH PCR analysis may be of added value for clonality assessment.
This master mix targets conserved regions within the variable (VH), diversity (DH), and the joining (JH) regions that flank the unique hypervariable, antigen-binding, complementarity determining region 3 (CDR3) and contains seven Framework 2 primers and a consensus JH primer. Clonality is indicated if the master mix generates clonal band(s). PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium staining.
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This product is for Research Use Only; not for use in diagnostic procedures.
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