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CAT #: 11010010

IGH Gene Rearrangement Assay for Gel Detection

Assay Use

This Research Use Only assay identifies immunoglobulin heavy chain clonality and is useful for the study of identifying clonal B-cell populations and evaluation of new research and methods in malignancy studies.

Product Details

  • Summary and Explanation of the Test

    BACKGROUND:

    Rearrangements of the antigen receptor genes occur during ontogeny in B and T lymphocytes.  These gene rearrangements generate products that are unique in length and sequence for each cell.  Therefore, polymerase chain reaction (PCR) assays can be used to identify lymphocyte populations derived from a single cell by detecting the unique V-J gene rearrangements present within these antigen receptor loci.1  This PCR assay employs multiple consensus DNA primers that target conserved genetic regions within the immunoglobulin heavy chain gene.  This test is used to detect the vast majority of clonal B-cell malignancies from DNA.  Test products can be analyzed using a variety of detection formats, including gel and capillary electrophoresis.

    SUMMARY:

    Invivoscribe’s gene rearrangement and translocation assays represent a simple approach to PCR-based clonality testing.  These standardized assays were carefully optimized testing positive and negative control samples using multiplex master mixes.  This test kit includes four (4) master mixes.  IGH Framework 1 (FR1), IGH Framework 2 (FR2), IGH Framework 3 (FR3) master mixes target the framework 1, 2, and 3 regions (respectively) within the variable (VH) region and the joining region of the Ig heavy chain locus (Figure).  The last master mix, the Amplification Control, targets the HLA-DQα locus and generates a product of 235 base pairs (bp) to ensure that the quality and quantity of input DNA is adequate to yield a valid result.

  • Specimen Requirements

    This assay tests genomic DNA.

References

1. Miller, JE et al. (1999). Molecular Diagnostics. 4:101-117.

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