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CAT #: 21010080

IGH Framework 3 (FR3) - Unlabeled for Gel Detection

Product Use

IGH Framework 3 (FR3) – Unlabeled for Gel Detection is a Research Use Only master mix that can be used to identify immunoglobulin heavy chain clonality.

Product Details

  • Description

    Genes encoding immunoglobulin heavy chain (IGH) molecules are assembled from multiple polymorphic gene segments that undergo rearrangement and selection during B-cell development.  Rearrangement of these variable (V), diversity (D), and joining (J) genetic segments result in VDJ products of unique length and sequence.  Clonal IGH rearrangements can identified through analyses of the size distributions of DNA products amplified from conserved sequences that flank this region.  For example, DNA isolated from a normal polyclonal population of B-cells generates a Gaussian distribution (bell-shaped size curve) of amplified products, whereas DNA amplified from a clonal B-cell population generates one or two product(s) of unique size(s) that reflect proliferation of a single rearranged clone.

    This master mix amplifies gDNA, targeting the conserved Framework 3 (FR3) region of the IGH gene and the joining (JH) region.  These regions flank the unique, hypervariable, antigen-binding, complementarity determining region 3 (CDR3).  Clonality is indicated if the master mix generates clonal band(s).  PCR products can be analyzed using standard gel electrophoresis using heteroduplex analysis with ethidium bromide staining.

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