CAT #: 24120011
FLT3 ITD Master Mix - 6FAM & HEX for ABI Fluorescence Detection
FLT3 ITD – 6FAM & HEX for ABI Fluorescence Detection is a Research Use Only master mix that can be used to identify FLT3 ITD mutations.
FLT3 is a receptor tyrosine kinase that is normally expressed on many cell types including hematologic stem cells. Mutation of the FLT3 receptor, by either internal tandem duplication (ITD) of the juxtamembrane domain or point mutation in the activation loop of the tyrosine kinase domain (TKD), causes constitutive activation of the FLT3 receptor. The most prevalent type of FLT3 mutation is an internal tandem duplication in and around the juxtamembrane domain. The second most common mutation type in the FLT3 gene is a TKD point mutation in aspartate (D835) or isoleucine (I836).
Detection of the ITD mutation can be determined based on the increased size of the PCR products compared to the wild-type product. The presence of a FLT3 ITD mutation is indicated if the amplicon is larger than 330 bp. PCR products are differentially labeled with 6FAM & HEX and can be analyzed using any platform that has the ability to detect these fluorochromes.
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