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CAT #: 13090030

BCL2/JH t(14;18) Translocation Assay MegaKit - Gel Detection

Product Use

BCL2/JH t(14;18) Translocation Assays are useful for the study of:

  • Monitoring and evaluation of follicular lymphomas and other B cell lymphomas
  • Distinguishing lymphoma from benign lymphoid hyperplasia
  • Distinguishing follicular lymphoma from other B cell lymphomas that may have a similar appearance
  • Monitoring and evaluation of disease recurrence
  • Detection and assessment of residual disease
  • Evaluation of new research and methods in malignancy studies

Product Details

  • Summary of Explanation of the Test

    BACKGROUND:

    BCL2 translocations are reciprocal chromosome exchanges that place the bcl-2 proto-oncogene, located on chromosome 18, under aberrant transcriptional control of the immunoglobulin heavy chain gene, located on chromosome 14. The bcl-2 protein is an antagonist to apoptosis (programmed cell death), a normal process designed to eliminate unneeded and damaged cells during hematopoiesis. Increased expression of the bcl-2 protein leads to an increase in the levels of B cells in the body.

    BCL2 t(14;18) translocations are present in 70-90% of follicular non-Hodgkin B cell lymphomas, 50% of undifferentiated B cell lymphomas, and 20-30% of large cell diffuse B cell lymphomas. They are not seen in other lymphomas; therefore, this test is useful for the differential findings of B cell malignancies. Further, presence of the bcl-2 translocation is an indicator of poor prognosis in large cell diffuse B cell lymphomas.

    This BCL2 t(14;18) Translocation Assay contains five master mixes. Primers in four of the master mixes target the joining region of the immunoglobulin heavy chain gene, and distinct regions of the BCL2 gene. These master mixes are used to detect BCL2 t(14;18) translocations. The remaining master mix targets a ubiquitous HLA class II gene to ensure DNA of sufficient quality and quantity was present to generate a valid result. Positive and negative control DNAs are also included. This assay is used both to identify and monitor BCL2 translocations involving the major breakpoint region (Mbr) or the minor cluster region (mcr), and to detect residual or disease recurrence. The limit of detection using nested amplification is less than one BCL2 translocation positive cell in ten thousand normal cells (limit of detection <10-4).

  • Specimen Requirements

    This assay tests genomic DNA

    • 5cc of peripheral blood, bone marrow biopsy, or bone marrow aspirate anti-coagulated with heparin or EDTA. Ship at ambient temperature; OR
    • Minimum 5mm cube of tissue shipped frozen; or at room temperature or on ice in RPMI 1640; OR
    • 2µg of genomic DNA; OR
    • Formalin-fixed paraffin embedded tissue or slides.

Disclaimer

This product is sold FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.

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