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CAT #: 20960011

Amplification Control - 6FAM for ABI Fluorescence Detection

Assay Use

The General Purpose Reagent (GPR).  Amplification Control master mix can be used to ensure that the quantity and quality of unknown sample DNA is adequate to yield a valid result during molecular testing.

Product Details

  • Summary and Explanation of the Test

    Sample quality is critical and therefore it must be verified before the samples are used in molecular assays.  Analysis of degraded DNA samples can result in poor quality data, and the presence of inhibitors differentially affects qPCR assays.1,2  Therefore, verification of the quantity and quality of the sample is sufficient for the molecular assay is important, and one method is to employ an amplification control master mix.

    Master mixes are components of complete assays and typically consist of a buffered magnesium solution, deoxynucleotides, and multiple primers that target the gene segments of interest.  Multiple primers are used to ensure a more comprehensive testing approach necessary to reliably identify clonal rearrangements.  This test is complete with the exception of Taq DNA polymerase, which is not provided.

    The Amplification Control master mix is included in many of our testing kits and targets the HLA-DQα locus; the Major Histocompatibility Complex or Human Leukocyte Antigen (HLA), class II, DQ alpha 1.  Amplification of genomic DNA (gDNA) with this master mix produces a product of 235 base pairs (bp) to ensure that the quantity of the sample DNA is adequate to yield a valid result.

  • Specimen Requirements

    This master mix tests genomic DNA (gDNA) from the following sources:

    • 5 cc of peripheral blood, bone marrow biopsy or bone marrow aspirate anti-coagulated with heparin or EDTA. Ship at ambient temperature; OR
    • Minimum 5 mm cube of tissue shipped frozen; or at room temperature or on ice in RPMI 1640; OR
    • 2 µg of gDNA; OR
    • Formalin-fixed paraffin embedded tissue or slides

References

1.  Vermeulen J et al. (2011) Nucleic Acids Research. May;39(9):e63.
2.  Huggett JF et al. (2008) BMC Research Notes. Aug 28;1:70.

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