Historically, T Cell Receptor Beta Chain Gene Rearrangement Assays have been used to:
- Identify clonality highly suggestive of T-cell and some immature B-cell malignancies
- Determine lineage involvement in mature lymphoproliferative disorders
- Monitor and evaluate disease recurrence
Scribe Technologies' assay are standardized PCR-based tests. Each test comes with a Standard Operating Procedure (SOP), an interpretation guide, master mixes, and controls. Master mixes are composed of a buffered magnesium solution, deoxynucleotides, and multiple primers that target the gene segments of interest. Multiple primers are used to ensure a more comprehensive testing approach necessary to reliably identify clonal rearrangements. These tests are complete with the exception of Taq DNA Polymerase, which is not provided. A single thermocycler program and similar detection methods are used within each series of test to improve consistency, reduce human error, and facilitate cross training.
Standard Protocol. Click to read more
This product tests genomic DNA. DNA can be extracted from the following specimens:
- 5cc of peripheral blood, bone marrow biopsy, or bone marrow aspirate anti-coagulated with heparin or EDTA; OR,
- Minimum 5mm cube of tissue shipped frozen, at room temperature, or on ice in RPMI 1640; OR,
- Formalin-fixed paraffin embedded tissue or slides.