All of our DNA controls are adjusted to a final concentration of 200 ug/ml in 1/10 TE (1mM Tris-HCl (pH8.0), 0.1mM EDTA). This diluent provides sufficient buffering capacity and EDTA to protect the DNA controls without interfering with the Mg concentrations required for robust amplification reactions.
DNA sensitivity panels are 6 member panels that consist of 100% clonal DNA extracted from a clonal cell line and 30%, 20%, 10%, 5% and 1% dilutions of the clonal DNA diluted (v/v) into our standard negative control DNA, IVS-0000 Polyclonal Control DNA, cat# 4-092-0010.