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DNA Controls
DNA controls can be used either as positive or negative controls for an assay depending on their genetic make-up. Please see the specific DNA control product page for their possible applications.
These controls yield positive results when used in the appropriate IVS Gene Rearrangement, Translocation, or Mutation Assays.
Sensitivity panels consist of a 100%, 30%, 20%, 10%, 5%, and 1% dilution of the indicated control.
Plasmid DNA controls are engineered to test positive for a specific chromosome translocation or mutation using our assay master mixes. The plasmid is cloned in standard bacterial culture conditions recommended by the supplier. Our controls are tested extensively to ensure quality and reproducibility of your test results. Our plasmid DNA controls, including sensitivity controls and proficiency panel samples, are supplied in aliquots of 100 µl. Plasmids IVS-P001 and IVS-P003 are diluted in 1/10 TE (1 mM Tris-HCl pH 8.0, 0.1 mM EDTA). This diluent provides sufficient buffering capacity and EDTA to protect the DNA controls without interfering with the Mg+2 concentrations required for robust amplification reactions. IVS-P002 is diluted in IVS-0000 Polyclonal Control DNA to mimic a normal background population.
Plasmid DNA controls are engineered to test positive for a specific chromosome translocation or mutation using our assay master mixes. The plasmid is cloned in standard bacterial culture conditions recommended by the supplier. Our controls are tested extensively to ensure quality and reproducibility of your test results. Our plasmid DNA controls, including sensitivity controls and proficiency panel samples, are supplied in aliquots of 100 µl. Plasmids IVS-P001 and IVS-P003 are diluted in 1/10 TE (1 mM Tris-HCl pH 8.0, 0.1 mM EDTA). This diluent provides sufficient buffering capacity and EDTA to protect the DNA controls without interfering with the Mg+2 concentrations required for robust amplification reactions. IVS-P002 is diluted in IVS-0000 Polyclonal Control DNA to mimic a normal background population.